Facile synthesis of covalent probes to capture enzymatic intermediates during E1 enzyme catalysis.
نویسندگان
چکیده
We report a facile synthetic strategy to prepare UBL-AMP electrophilic probes that form a covalent bond with the catalytic cysteine of cognate E1s, mimicking the tetrahedral intermediate of the E1-UBL-AMP complex. These probes enable the structural and biochemical study of both canonical- and non-canonical E1s.
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ورودعنوان ژورنال:
- Chemical communications
دوره 52 12 شماره
صفحات -
تاریخ انتشار 2016